H3R17me2 polyclonal antibody
产品名称： H3R17me2 polyclonal antibody
英文名称： H3R17me2 polyclonal antibody
产品编号： PAB14059
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of H3R17me2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the asymmetrically dimethylated arginine 17 (H3R17me2 (asym)).
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In serum (0.05% sodium azide)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000-1:3000)
Dot Blot (1:20000)
Western Blot (1:250)
ChIP (10-15 ul/ChIP)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.Mol Cell. 2007 Jan 12;25(1):1-14.
- 2.
- Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28.
Dunn KL, Davie JR.Oncogene. 2005 May 12;24(21):3492-502.
- 3.
- Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.J Biol Chem. 2003 Jun 27;278(26):24217-24. Epub 2003 Apr 15.
- Applications
- ChIP
- ChIP assays were performed using human osteosarcoma (U-2 OS) cells, the polyclonal antibody against H3R17me2(asym) (Cat # PAB14059) and optimized PCR primer sets for qPCR.
Figure A: qPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene.
Figure B: qPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene.
